Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------MNNIGEIISNFEGIIGALLG--VIVTLILTHI-LKHFGQIKFYIVDFEIYFKTDNDGWGTNVMPSKDEAKQIEIHSQIEIYNGAEIPKVLREIKFCFYKNTNLIVSVTPDDKATTEEFAEFGYYRDKLFNINLPSKQIIAINIIKFLNEKETKQVKKCNRVYLEAKDHNGKMYKVFLGEF------------------------------------------------------------------------------------------------------------------------------------------------ 5I0P Chain:A ((13-366)) EHQLDYPFADTLPAAGDTFEVAPGVRWLRMPLPFSLDHINLWLLRDEIDGQAGWTIVDCGISS-EAIRTHWEQIFDRHLDGLPVLRVLVTHCHPDHFGLANWLCEGG------DQGRWNVRLWMTLGEYMFGCLMA----------------------------------AGAAADHFARHGLYYSDLVPAVPPRY----------------RRLREGDAVKI-----GARTWRVVTGYGHSPEHCALHSEADGVLISGDMVLPRISTNVSVFDLEPEANPLALYLESLGRYETMAPDTLVLPSHGKPFRGVRTRIAQLRAHHDARLEEVRVACAEKPMSAAGIVPIMFTFALGEALAHLNLLWLAGELVREHGDDGVIRYARA

General information: TITO was launched using: RESULT: Template: 5I0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 487 -3819 -7.84 -33.21 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -7.84 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_5I0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I0P-query.scw PDB file : Tito_Scwrl_5I0P.pdb: