Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDI--------------FNIQTSDRMKELIDVLYERF-DDLSEDTKK----AVELAKKEYEENKK--------IPEAEYKEYVILC--SKAETAWEEAKGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEAIIPLVKQVTASGN---K-----PDT-----------------------SFITK------------AFPKEKQKELSLYFLQELGYDFDG-----------GRLDE--TVHPFATTLNR-GDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPYYKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDA----EGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKMLEDLP---EFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGEE-LNVRYLIDYLSNKYSNLYLL 5AMB Chain:A ((16-579)) ----GAQLFAQSYQSSAEQVLFQSVAASWAHDTNITAENARRQEEAALLSQEFAEAWGQKAKELYEPIWQQFTDPQLRRIIGAVRTLGSANLPLAKRQQYNALLSQMSRIYSTAKVCLTATCWSLDPDLTNILASSRSYAMLLFAWEGWHNAAG-IPLKPLYEDFTALSNEAYKQDGF-TDTGAYWRSWYNSPTFEDDLEHLYQQLEPLYLNLHAFVRRALHRRYGDRYINLRGPIPAHLLGDMWAQSWENIYDMVVPFPDKPNLDVTSTMLQQGWQATHMFRVAEEFFTSLELSPMPPEFWEGSMLEKPADGREVVCHASAWDFYNRKDFRIKQCTRV--TMDQLSTVHHEMGHIQYYLQY-KDL-PVSLRRGANPGFHEAIGDVLALS-VSTPEHLHKIGLL-DRVTND------TES---DINY----LLKMALEKIAFLPFGYLVDQWRWGVFSGRTPPSRYNFDWWYLRTKYQGICPPVTRNETHFDAGAKFHVPN--VTPYIRYFVSFVLQFQFHEALCKEAGYEGPLHQCDI-YRSTKAGAKLR-KVLRAGSSRPWQEVLKDMVGLDALDAQPLLKYFQLVTQ-----

General information: TITO was launched using: RESULT: Template: 5AMB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2079 16627 8.00 35.60 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 8.00 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_5AMB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AMB-query.scw PDB file : Tito_Scwrl_5AMB.pdb: