Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKNITLTILLTNLFIAFLGIGLVIPVTPTIMNELH--------------LSGTAVGYMVACFAITQLIVSPIAGRWVDRFGRKIMIVIGLLFFSVSEFLFGI---GKTVEMLFISRMLGGISAAFIMPGVTAFIADITTIKTRPKALGYMSAAISTGFIIGPGIGGFLA--E-VHSRLPFFFAAAFALLAAILSILTLREPERNPEN---QEI-------------------------------KGQKTGFKRIFAPMYFIAFLIILISSFG-----LASFESLFALFVDHKFGFTASDIAIMITGGAIVGAITQVVLFDRFTRWFGEIHLIRYSLILSTSLVFLLTTVH---------SYVAI-LLVTVTVFVGFDLMRPAVTTYLSKIAGN-EQGFAGGMNSMFTSIGNVFGPIIGGMLFDIDVNYPFYFATVTLAIGIALTIAWKAPAHLKAST 4ZW9 Chain:A ((46-449)) -----------------FGYNTGVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAAL-PFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKD---AGVQEPIYATIGAGVVNT-IFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAF-FEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYL--------------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1241 -153047 -123.33 -458.22 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -123.33 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: