Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTT----NLKEFISLLHPPRILWVMVPHGI-VDAVLRDVSPLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAV-----ENGYLYAGEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARV---WNHGSVIRSWLMGLTERAFAKDAK-----LDQIKGIMHSSGEGKWTVETALDLQTATPVIAMSLMMRY-RSLTDDTFTG-KVVAALRNEFGGHATEKK 3FWN Chain:B ((17-328)) -QIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGG-LNLTNEELAQTFTEWNNGE-LSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAE--

General information: TITO was launched using: RESULT: Template: 3FWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1419 11367 8.01 38.93 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 8.01 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_3FWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FWN-query.scw PDB file : Tito_Scwrl_3FWN.pdb: