Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGFFDSGIGGMTVLYEAIKVLPYEDYIFYADTLNVPYGEKSKGKVKEYIFNAAEFLASQNIKALVIACNTATSIAIEDLRRNFDFPIIG-IEPAVKPAINKCTEERKRVLVVATNLTLKEEKFHNLVKEIDHHD---LVDCLALPGLVEFAENFDFSEDKIIKYLKNELSSFDLKQYGTIVLGCTHFPFFKNSFEKLFGIKVDMISGSVGTAKQLKKVLADRNQLGKGSGSITFFNSGHKIVDQEVISKYKRLFEILDETQRSHVGH 3UHO Chain:B ((25-237)) MKIGVFDSGVGGLSVLKSLYEARLFDEIIYYGDTARVPYGVKDKDTIIKFCLEALDFFEQFQIDMLIIACNTASAYALDALRAKAHFPVYGVIDAGVEATIKALHDKNKEILVIATKATIKSEEYQKRLLSQGYTNINALATGLFVPMVEEGIFEGDFLQSAMEYYFKN-ITTPD-----ALILACTHFPLLGRSLSKYFGDKTKLIHSGDAIVEFLKE--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1188 -142341 -119.82 -681.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -119.82 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_3UHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UHO-query.scw PDB file : Tito_Scwrl_3UHO.pdb: