Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKIVANTADLIGDTPLVRLNRLQPENAAQVYLKLEFFNPSGSVKDRAAYQMIIEAEQNGLLKPGSVIIEPTSGNTGIGLAMNAAARGYKAILVMPDTMTKERINLLKAYGAEVVLTPGEERMPGSIKKAKELAEQIPNSYIPMQFDNTANPDAHRKTTAPEIARAIEEIGKPLGAFVASSGTGGTITGTGEALKELFPDITVHVVEPAGSPVLSGGKPGAHKLVGTSPGFIPPILNEDVYDEIIKISDEDAYTTTRRLAAEEGILVGPSSGAACFAAIETAKR-LSPDKVVVCMTADTGERYLSTDLWSFI 4I1Y Chain:B ((5-301)) -MTIAENIAQLIGGTPLVRLRRVTDGAAADVVAKLESFNPAGSIKDRIGVAMIDAAEKAGLIKPDTIILEPT--NTGIALAMVSAARGYKCVLTMPDTMSIERRMLLRAYGAELVLTPGAEGMAGAIAKAEELAKTDDRYFIPQ------NPAVHAVTTAEEVWRDTD--GK-VDIFVSGVGTGGTITGVAQVIKQRRPSAQFVAVEPA--------------------------LDLALVDEVITVGNDDALELARRMATEEGLLFGISSGAAVWAARELAHRPENAGKLIVVVLPDFGE-----------

General information: TITO was launched using: RESULT: Template: 4I1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1451 -151155 -104.17 -576.93 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -104.17 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_4I1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I1Y-query.scw PDB file : Tito_Scwrl_4I1Y.pdb: