Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFCTFFEKHHRKWDILLEKSTGVMEAMKVTSEEKEQLSTAIDRMNEGLDAFIQLYNESEIDEPLIQLDDDTAELMKQARDMYGQEKLNEKLNTIIKQILSISVSEEGEKE 2MFL Chain:A ((87-179)) ----EKAKRHEAW-ITLEKAYE--DAETVTGVINGKVKGGFTVELNGIRAFLP---GSLVDVRPVR---DTLHL-EGKELEFKVIKLDQKRNNVVV---SRRAVIESENS

General information: TITO was launched using: RESULT: Template: 2MFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -6095 -18.58 -65.53 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -18.58 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.119

(partial model without unconserved sides chains): PDB file : Tito_2MFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MFL-query.scw PDB file : Tito_Scwrl_2MFL.pdb: