Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MIPIPSAIDGQSFLLQELEQVMKPLGYVINGGWEYDHG-YFDYKIDDRDGYLFLRIPVNAVQGSLDERGAAVRIG-TPFMLRQVFQADVDDHAEGGPFQSLFNQFSEPERRDAEIDPAFLDIGASLVKELEDVLLH 2PWW Chain:A ((2-116)) SLKFPDTGLEEKEVAFSIVNHAAKSLGFIHVDQWDYERVMF-DYKIVHHEGTFYLRVPAYAVKGEIPRPSTIVQI-MTPILGKYYYPHGVEYEGETFP-------------------QAVIDKCNNKLALLAKTIK-

General information: TITO was launched using: RESULT: Template: 2PWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 458 -18921 -41.31 -168.94 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -41.31 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_2PWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PWW-query.scw PDB file : Tito_Scwrl_2PWW.pdb: