Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKA----FSSDVSVLALSISIYGVMIFIGAPLLVPLGDKYSRELSLLAGLMIFIIGTVICALAQN-------IFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVPLGSFIGGV--------------LHWRWTFWIFALMGVLVVLLILLEMRRHAQHKNSGKEEIEEPA-GTFRDALKV----PRVPVYITITFCNMIGFYGMYSFLGTYLQDVFTGGNTA----------AGLFIMIYGIG-FSMSVITGKIADRIGKMRSLLIALGVISVLLACLPY-APA-S-MFLLIASLFIWGLMQSLTVTLLSTILSDCSE-RHRGKVMVFYSLASNLAVTLGSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYGKLEQKADQSLSQ 4J05 Chain:A ((35-498)) -----------------KLVLLAGVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQ-R---------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQ-IGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIGTSAVLWIFFSTCILGFIST--------------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1653 -221784 -134.17 -630.07 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -134.17 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.324

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: