Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKSYLITGGAGFIGLTFTKLMLRE-TDARITVLDKLTYASHPEEMEKLKQNSRFRFVKGDISVQEDIDRAFDETYDGVIHFAAESHVDRSISQAEPFITTNVMGTYRLAEAVLKGKAKKLIHISTDEVYGDL--KADDPA--------FTETTPLSPNNPYSASKASSDLLVLSYVKTHKLPAIITRCSNNYGPYQHSEKMIPTIIRHAKQGLPVPLYGDGLQIRDWLFAEDHCRAIKLILEKGTDGEVYNIGGGNERTNKELASVILKHLG-CEELFAHVEDRKGHDRRYAINASKLKNELGWRQEVT-FEEGIARTIQWYTDNDR 1KEP Chain:B ((5-325)) --KNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNKANLEAI-LGDRVELVVGDIADAELVDKLAAKA-DAIVHYAAESHNDNSLNDPSPFIHTNFIGTYTLLEAARKYDIR-FHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSPYSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKDAYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSEGLEETIQWYTDN--

General information: TITO was launched using: RESULT: Template: 1KEP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1689 32125 19.02 104.30 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 19.02 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_1KEP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KEP-query.scw PDB file : Tito_Scwrl_1KEP.pdb: