Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQIIFGAGV-VNKIYKEVVHVWETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAI--TASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILL--PILVGFSAARQFGSNPYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSIVPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGI-YSTIVLSGLHHSFYAI-EATLLANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKIALPASLTAFLGIV--EPIVFGVNLKLIRPFIGAAIGGAIGGAYVVAV-------QVVANSYGLTGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATL--------FLGFKEETE--------- 4C1N Chain:A ((1-442)) GLTGLEIYKHLPKKNCKECG-QPTCLAFAMQIAAGKAGLDACPYVSDEAKELLESASAP----PVALIKVGKGEKVLEIGHETVLFRHDKRFEHPCGLAILVEDTLSEGEIKERVEKINKLVFDRVGQMHSVNLVALKGSSQDAATFAKA---VATAREVTDLPFILIGTPEQLAAALETEGANNPLLYAA----------------------TADNYEQMVELAKKYN--VPLTVSAKGLDALAELVQKITALGYKN----------------LILDPQP----ENISEGLFYQTQIRRLAIKKLFRPFGYPTIAFALDENPYQAVMEASVYIAKYAGIIVLNTV----EPADILPLITLRLNIYTDPQKPIAVEPKVYEILNPGPDAPVFITTNFSLTYFCVAGDVEGARIPAYILPVDTDGTSVLTAWAAGKFTPEKIAQFLKESGIAEKVNHRKAILPGGVAVLSGKLQELSGWEILVGPRESSGINSFIKQRW

General information: TITO was launched using: RESULT: Template: 4C1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2042 -76294 -37.36 -189.32 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -37.36 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_4C1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C1N-query.scw PDB file : Tito_Scwrl_4C1N.pdb: