Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFVSMKELLEDAKREQYAIGQFNINGLQWTKAILQAAQKEQSPVIAAASDRLVDYLGGFKTIAAMVGALIEDMAITVPVVLHLDHGSSAERCRQAIDAGFSSVMIDGSHQPIDENIAMTKEVTDYAAKHGVSVEAEVGTVGGMEDGLVGGVRYADIT---ECERIVKETNIDALAAALGSVHG--KYQGEPNLGFKEMEAISRMTDIPLVLHGAS---------------------GIPQDQIKKAITLGHAKININTECMVAWTDETRRMFQENSDLYEPRGYLTPGIEAVEETVRSKMREFGSAGKAAKQQVG 3N9S Chain:B ((2-306)) --LVKGNEILLKAHKEGYGVGAFNFVNFEMLNAIFEAGNEENSPLFIQASEGAIKYMG-IDMAVGMVKIMCERYP-HIPVALHLDHGTTFESCEKAVKAGFTSVMIDASHHAFEENLELTSKVVKMAHNAGVSVEAELGRLM------------AVLVNPKEAEQFVKESQVDYLAPAIGTSHGAFKFKGEPKLDFERLQEVKRLTNIPLVLHGASAIPDNVRKSYLDAGGDLKGSKGVPFEFLQESVKGGINKVNTDTDLRIAFIAEVRKVANEDKSQFDLRKFFSPAQLALKNVVKERMKLLGSANK-------

General information: TITO was launched using: RESULT: Template: 3N9S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1432 -96241 -67.21 -360.45 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -67.21 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_3N9S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N9S-query.scw PDB file : Tito_Scwrl_3N9S.pdb: