Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQV--DLQ--PEDYHVYWNYAV-KKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLEA-GFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI 4LND Chain:C ((30-286)) LKICSWNVDGLRAWIKKKG-LDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 4LND.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1368 12617 9.22 50.26 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain C : 0.87 3D Compatibility (PKB) : 9.22 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_4LND.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LND-query.scw PDB file : Tito_Scwrl_4LND.pdb: