Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSISNITVYMPSVLTLVGIPGLESVQCWIGIPFCAIYLIAMIGNSLLLSIIKSERSLHEPLYIFLGMLGATDIALASSIMPKMLGIFWFNVPEIYFDSCLLQMWFIHTLQGIESGILVAMALDRYVAICYPLRHANIFTHQLVIQIGTMVVLRAAILVAPCLVLIKCRFQFYHTTVISHSYCEHMAIVKLAAANVQVNKIYGLFVAFTVAGFDLTFITLSYIQIFITVFRLPQK--------EARFKAFNTCIAHICVFLQFYLLAFFSFFTHRFGS-HISPYIHILFSSIYLLVPPFLNPLVYGAKTTQIRIHVVKMFCS 1U19 Chain:A ((41-323)) --------------------------SMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRF-GENHAIMGVAFTWVMALACAAPPLVGWS-RYIP----EGMQCSCGIDYYTPH---EETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCC

General information: TITO was launched using: RESULT: Template: 1U19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -270391 for 2221 contacts (-121.7/contact) + 2D Compatibility (PS) -27401 + (NN) 255 + (LL) 2788 1D Compatibility (HY) -4400 + (ID) 900 Total energy: -300049.0 ( -135.10 by residue) QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_1U19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U19-query.scw PDB file : Tito_Scwrl_1U19.pdb: