Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNIKSDRIMTSDPQKFTVDGVRFFQQYLNKIRPDAMEIDNLIINSDIIKEDLIVHVSKMIDRLALYDLHRKRSIDKHF----SAAFTVDINDREEFYSANIADVSIDLNESSGV------------------------ 4FC8 Chain:A ((1-152)) MATIEEIKEVVLKPYT------NHRQLTIREVETISINLIDLLITKDVKDARTMKYISRFLTKQDYADLVQERNLVKRCGYPLCSKSQARVAYLTEYCTKAHFRCSQFYQFQLSDEALFARVGVHLDDYEPPSEIQLLE

General information: TITO was launched using: RESULT: Template: 4FC8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 335 -30313 -90.49 -288.69 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -90.49 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_4FC8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FC8-query.scw PDB file : Tito_Scwrl_4FC8.pdb: