Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPYIILEDQTRLYYETHGSGTPILFIHGVLMSGQFFHKQFSVLSANYQCIRLDLRGHGESDKVLHGHTISQYARDIREFLNAMELDHVVLAGWSMGAFVVWDYLNQFGNDNIQAAVIIDQSASDYQWEGWEHGPFDFDGLKTAMHAIQTDPLPFYESFIQNMFAEPPAETETEWMLAEILKQPAAISSTILFNQTAADYR-GTLQNINVPALLCFGEDKKFFSTAAGEHLRSNIPNATLVTFPKSSHCPFLEEPDAFNSTLLSFLDGVIGKS 3BF8 Chain:A ((1-255)) ----MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHNIIQVDVRNHGLSPRE-PVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA-PDRIDKLVAIDIAPVDYH-------VRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQFLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLLAQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLNDH----

General information: TITO was launched using: RESULT: Template: 3BF8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1418 -196854 -138.83 -775.02 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -138.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3BF8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BF8-query.scw PDB file : Tito_Scwrl_3BF8.pdb: