Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTENFWRELPRPFFVLAP-MEDVTDVVFRHVVSEAGRPDVFFTE-FTNSESYCHPDG-KDSVRGRLTFTEDEQPIVAHIWGDKPENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELIQAAKAG-GLPVSVKTRLGYTDVDEWREWLTHILKQDIANLSIHLRTRAEMSKVDAHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPFAFEKEPKEHS--------SKELLDLLRLHLDLHDEYSKEEARPYK-PLPRFFKIYLRGFRGASELRNQCM-NTKSTDEVRA-LLDDFERKYLDGIE 1VHN Chain:A ((5-309)) -----------VKVGLAPMA-GYTDSAFRTLAFEWG-ADFAFSEMVSAKGFL-----MNSQKTEELLPQPHERNVAVQIFGSEPNELSEAARILSE-KYKWIDLNAGCPVRKVVKEGAGGALLKDLRHFRYIVRELRKSVSGKFSVKTRLGWEK-NEVEEIYRILVEEGVDEVFIHTRTVVQSFTGRAEWKALSVLEK-------RIPTFVSGDIFTPEDAKRALEESGCDGLLVARGAIGRPWIFKQIKDFLRSGKYSEPSREEILRTFERHLELLIKTKGERKAVVEMR--KFLAGYTKDLKGARRFREK-VMKIEEVQILKEMFYNFIKEVE-----

General information: TITO was launched using: RESULT: Template: 1VHN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1568 -144805 -92.35 -499.33 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -92.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1VHN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHN-query.scw PDB file : Tito_Scwrl_1VHN.pdb: