Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKKAFILSAAAAVGLFTFGGVQQASAKELSCQPVVTVKTGNTVQNMSLNDAVKKLHINT---NIKTLNAANEK----------EMKQLLQKHAKQSNVKVQ------DVQKTETAKPAQKTTE--------------------KAAADQ-NTASKAPATAEKTNTTTSAPSSVSAYEKKVVELTNAE---RQKQGLKPLQIDETLSKSARAKSQDMKDKNYFDHQSPTYGSPFDMMKSFGISYKTAGENIAKGQKTPEEVVKAWM-NSEGHRKNILNPNFTH-IGVG-YVESGSIWTQQFIGK 4H0A Chain:A ((7-323)) FDVLENAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELSDEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNKRPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIAS-------NNLYEATSEFTEDALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSW-MNSDIHRSRLLNSKYDEMGGDVMR----DYYSLIFLEK

General information: TITO was launched using: RESULT: Template: 4H0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1083 13691 12.64 55.88 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 12.64 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_4H0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0A-query.scw PDB file : Tito_Scwrl_4H0A.pdb: