Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------MKNILR-------AMVILLIICGTYVLFIQYGSVPE-------KKSNDSEPQVSNEEAQSGKRIHMPTSGLLSFMGKH-ADEVTKKLGEPERIDPSAYDYDWWVYNQG------KDQYIQIGVLNNKVVTLFAS-GNDINAKPFKIGESTGEVFKTTQVAPFVNVEYKGNSYRFEFSEEDINTRPT--------VKVGKMYVQLYM------------------DKFEGKLSSIRA--FDAQTFVKQRPYEVVYRGELIKPKAVSDEKWKKIQTTSEKQIL-DLTNVIRVK-HGLAKLEWDQPTAEVAFGHSEDMKEN---------------------------------NYFSHVSKKYGSLKDRLEEGH------VDFQQAGENIAYNYVDGPAAVEGWLNSEGHRKALLNSDYTHLGVGVDRKYYT-QNFIKRW------- 4ZH0 Chain:A ((27-527)) QDLSDRYESLNNLLNRYSTLNTLIKLSADPSAINAVRENLGASAKNLIGDKANSPAYQAVLLAINAAVGFWNVVGYVTQCGGNANGQKSISSKTIFNNEPGYRSTSITCSLNGHSPGYYGPMSIENFKKLNEAYQILQTALKRGLPALKENNGKVNVTYTYTCSGDGNNNCSSQVTGVNNQKDGTKTKIQTIDGKSVTTTISSKVVDSRADGNTTGVSYTEITNKLEGVPDSAQALLAQASTLINTINNACPYFHAPKFSTTTGKICGAFSEEISAIQKMITDAQELVNQT--SVINEHEQTTPVGNNNGKPFNPFTDASFAQGMLANASAQAKMLNLAEQVGQAINPERLSGTFQNFVKGFLATCNNPSQGSAPGTVTTQTFASGCAYVGQTITNLKNSIAHFGTQEQQIQQAENIADTLVNFKSRYSELGNTYNSITTALSNIPNAQSLQNAVSKKNNPYSPQGIDTNYYLNQNSYNQIQTINQEL

General information: TITO was launched using: RESULT: Template: 4ZH0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1263 -10867 -8.60 -31.59 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -8.60 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.111

(partial model without unconserved sides chains): PDB file : Tito_4ZH0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZH0-query.scw PDB file : Tito_Scwrl_4ZH0.pdb: