Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKKDIIRLLETIAVYMELKGDNPFKVSAFRKAAAALEQDDRSLSEMD-----DMMSLSGIGKGTYSVIKEYIDEGKSSTLESLQKEVPEGLVPLLKLPGLGGKKIAKLYKELGVHDAESLKEACEQQKVQGLAGFGKKSEEKILQALGEAGKQPERFPIGYALRIAREIEEHLSQFTHIIKFSRAGSLRRARETVKDLDYIIATDHPAEVREQLLELPNIKSVIASGDTKVSVILSFEYETSVDFRLVTEEQFPTTLHHFTGSKDHNIKMRQIAKERGERISEYGVETVETGEIKTFPSEREFYAHFGLPLIPPEIRESGQEVETYSD--SIELIELGQIKGDLHMHSTWSDGAFSIREMAEACIKKGYQYMAITDHSQYLKVANGLTAERLKQQAKEIDALNAEFENFRILKGVEMDILPDGTLDYDDDVLAEMDIVIASIHSSFNQPEHVIMKRLETALTNKHVDIIAHPTGRLIGRRAGYEIDIDQLIELARKTNTALELNANPARLDLRTEHLMKANEQGVTLVINTDAHNIEMLDDMKTGVTAARKGWTETKNVLNARSLKDVEAFLKRND 3B0X Chain:A ((1-572)) MRNQELARIFEEIGLMSEFLGDNPFRVRAYHQAARTLYDLDTPIEEIAEKGKEALMELPGVGPDLAEKILEFLRTGKVRKHEELSRKVPRGVLEVMEVPGVGPKTARLLYEGLGIDSLEKLKAALDRGDLTRLKGFGPKRAERIREGLALAQAAGKRRPLGAVLSLARSLLEAIRALPGVERAELCGSARRYKDTVGDLDFLVASREGERAVEGFVRLPQVKEVYAKGKERATVFLK--NGLQVDLRVVPPESYGAGLQYLTGSAAHSIRLRALAQEKGLKLSEYGVFRG--EKRIAGETEEEVYAALGLPWIPPPLREDQGEVEAALEGRLPKLLELPQVKGDLQVHSTYSDGQNTLEELWEAAKTMGYRYLAVTDHSPAVRVAGGPSPEEALKRVGEIRRFNETHGPPYLLAGAEVDIHPDGTLDYPDWVLRELDLVLVSVHSRFNLPKADQTKRLLKALENPFVHVLAHPTARLLGRRAPIEADWEAVFQKAKEKGVAVEIDGYYDRMDLPDDLARMAYGMGLWISLSTDAHQTDHLRFMELAVGTAQRAWIGPERVLNTLDYEDLLSWLKAR-

General information: TITO was launched using: RESULT: Template: 3B0X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3096 162013 52.33 286.75 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 52.33 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_3B0X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B0X-query.scw PDB file : Tito_Scwrl_3B0X.pdb: