Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAID--ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAM----DYLIKHPE-IKHGTIRVAFTPDEEIGR--GPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKIAMKLNSLLPADEAPEYTEGYEGFYHLLSIQGD------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDGSQLSYMGLPTPNIFTGGE-NFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 2RB7 Chain:A ((5-360)) -MQHIVELTSDLIRFPSMHSRP-------EQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVL-PEK---GRAGLLLMAHIDVVDAED---------DLFVP-RVE----------------------NDRLYGRG----ANDDKYAVALGLVMFRDRLNALKAAGRSQKDMALGLLITGDEEIGGMNGAAKALP-LIRADYVVALDGGNPQQVITKEKGIIDIKLTCTGKAAHGARP-WMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKVPDVAEGWFNIRVTEHDDPGALIDKIRKTVSGT--------VSIVR---TVPVFL--AADSPYTERLLALSGA-----TAGKAHGASDARYLGENGLTGVVWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEE--

General information: TITO was launched using: RESULT: Template: 2RB7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1906 31859 16.71 93.70 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 16.71 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_2RB7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RB7-query.scw PDB file : Tito_Scwrl_2RB7.pdb: