Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIKLEQLGYCYQKNSPFEKRALLDVNVSFDSGSYSAIIGHTGSGKSTLLQHLNALLMPTEGKITVGDREIVAGVKQKKLRDLRKKVGIVFQ----FPEAQLFEETVEKDICFGPMNF-GVSEEDAKLRAKKVIYEVGLTEEILSRSPFELSGGQMRRVAIAGVLAMDPEVLVLDEPTAGLDPHGREEIMEMFYNLHKEKGLTTVLVTHSMEDAARYAEKIVLMKAGTVLQIGTPREIFAKPDELVDLGLSVPDVVRFQGLFERKFDVKLTKTCLTIDELTTEMAPYLAKGGA 3C4J Chain:A ((43-250)) -------------------------INVHIREGEVVVVIGPSGSGKSTFLRCLNLLEDFDEGEIIIDGINLKA--KDTNLNKVREEVGMVFQRFNLFPHM-----TVLNNITLAPMKVRKWPREKAEAKAMELLDKVGLKDKAHA-YPDSLSGGQAQRVAIARALAMEPKIMLFDEPTSALDPEMVGEVLSVMKQLANE-GMTMVVVTHEMGFAREVGDRVLFMDGGYIIEEGKPEDLFDRP---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3C4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -113947 for 1584 contacts (-71.9/contact) + 2D Compatibility (PS) -21770 + (NN) -6579 + (LL) 6432 1D Compatibility (HY) -18400 + (ID) 3950 Total energy: -158214.0 ( -99.88 by residue) QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_3C4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C4J-query.scw PDB file : Tito_Scwrl_3C4J.pdb: