Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSAQPKADTQAYIQNWADQYSQYDRFLKGENAYVAGVLYDFLEKARASNVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAE------AREKAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKN-GAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 1Y8O Chain:A ((245-374)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEG-YPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFN-YMYS---PL--FGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKAL-

General information: TITO was launched using: RESULT: Template: 1Y8O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 433 -48423 -111.83 -452.55 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -111.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1Y8O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y8O-query.scw PDB file : Tito_Scwrl_1Y8O.pdb: