Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPAKTFVILCILFLLDLCFSYIRHEWHS-QNALQDMPVPSDLHPIVKQNADALKAAAANKGIDVV-ITEGFRSFKEQDELYKQGRTKKGN---IVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGF--EWGGDWKR----FKDYPHLEMIPN 4NT9 Chain:B ((52-193)) -----------DEIIVANKHYPLSK---DYNPG----------ENPTAKAELVKLIKAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFDVIGTDGD---------LVTEEKAAQWL--LDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVG-

General information: TITO was launched using: RESULT: Template: 4NT9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 541 -35005 -64.70 -267.21 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -64.70 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_4NT9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NT9-query.scw PDB file : Tito_Scwrl_4NT9.pdb: