Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISFSESQKLFAYFSGLIAALSLFIYYVSAQQSEGALILCITFGVIAAGIWFGPIYALAVTLIVLFVLGTLMMFFQTGQTSLFPAEEGLRMLVVWGIALLLFSFISGRIHDITAELRRSMTRLQSEIKSYVAVDRVTGFDNKQRMKLELSEEIKRAERYGNSFVFLLLHMHYFKEFKSLYGEKETDRLFQYVGQQIRTSVRETDKKFRPSDERIGIVLTHTPAEHMPAVLTKLKKQLDTYQLE--NGKYVSLTFHVCYLPYRNDIQTADQFLEELENEMMMNEL 4ZVF Chain:A ((1-148)) -------------------------------------------------------------------------------------------------------------------------------------MDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNG-HPDYERLIQIADEALYI---

General information: TITO was launched using: RESULT: Template: 4ZVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 618 -100367 -162.41 -687.45 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -162.41 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_4ZVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZVF-query.scw PDB file : Tito_Scwrl_4ZVF.pdb: