Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKITTNEQFN-ELIQSDKEIIVKFYADWCPDCTRMNMFIGDILEEYNQN-DWYELNKDELPDLAEKYQVMGIPSLLIFKNGEKTAHLHSANAKTPEEVTEFLSEHIS 2BTO Chain:T ((8-106)) -----TDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKG--QLKEFLDAN--

General information: TITO was launched using: RESULT: Template: 2BTO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 375 -61138 -163.03 -630.28 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain T : 0.78 3D Compatibility (PKB) : -163.03 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.659

(partial model without unconserved sides chains): PDB file : Tito_2BTO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BTO-query.scw PDB file : Tito_Scwrl_2BTO.pdb: