Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLGGIEAGGTKFVCAVGREDGTIIDRIEFPTK--MPDETIEKVIQYF----S--QFSLQAIGIGSFGPVDNDKTSQTYGTITATPK-AGWRHYPFLQTVKNEMKIPVGFSTDVNAAALGEFLFGEAKGLDSCLYITIGTGIGAGAIVEGRLLQGLSH--PEMGHIYIRRHPDDVYQGKCPYHG-DCFEGLASGPAIEARWGKKA------ADL--------SDIAQVWELEGYYIAQALAQYILILAPKKIILGGGVMQQ--KQVFSYIYQYVPKIMNSYLDFSELSDDISDYIVPPRLGSNAGIIGTLVLAHQALQAEAASGEVRS 3VOV Chain:A ((2-300)) KVVGLDLGGTKIAAGVFDG-KRLLSKVVVPTPKEGGERVAEALAEAAERAEREAGVRGEAIGLGTPGPLDFRR-----GVIR---NIPGVQDFPIRRILEEATGRPVFLENDANAAALAEHHLGAAQGEESSLYLTVSTGIGGGVVLGGRVLRGERGQGGELGHLTLLPG-----GPACGCGLEGCLEALAAGRALERDATYAFQRPVDTRELFRLFQAGDPKAERLVLQAARYVGIGLASLVKAFDPGVVVLGGGVALNAPEGYWEALLEAYRRYLQGW-E--------APPLRRARLGAEAGLLGAALTAYLEVKDG--------

General information: TITO was launched using: RESULT: Template: 3VOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1599 -198572 -124.19 -740.94 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -124.19 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3VOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VOV-query.scw PDB file : Tito_Scwrl_3VOV.pdb: