Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLMNEFEKACETLRKFMAYMLEK-DMKSWTELWDENAVFEFPYAPEGSPKRIEGKAAIYDYIKDYPKQIHLSSFTAPTVYRSADSNTVIAEFQCDGHVIETGLPYRQSYISVIETRDGRIVRYRDYWNPLVVKEAFGGSFLQTEESGK 4J9A Chain:D ((1-125)) --MNAKEIVVHALR-----LLENGDARGWSDLFHPEGVLEYPYPPPGYKTRFEGRETIWAHMRLFPEYMTIR-FTDVQFYETADPDLAIGEFHGDGVLTA-GGKLAYDYIAVWRTRDGQILLYRLFFNPLRVL---------------

General information: TITO was launched using: RESULT: Template: 4J9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 529 -57246 -108.21 -465.41 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -108.21 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4J9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J9A-query.scw PDB file : Tito_Scwrl_4J9A.pdb: