Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISVLFVCLGNICRSPMAEAIFRDLAAKKGLEGKIKADSAGIGGWHIGNPPHEGTQEILRREGISFDGMLARQVSEQDLDDFDYIIAMDAENIGSLRSMAGFKNTSHIKRLLDYVEDSDLADVPDPYYTGN--FEEVCQLIKTGCEQLLASIQKEKQL 3JS5 Chain:A ((22-175)) -MKLLFVCLGNICRSPAAEAVMKKVIQNHHLTEKYICDSAGTCSYHEGQQADSRMRKVGKSRGYQVDS-ISRPVVSSDFKNFDYIFAMDNDNYYELLDRCPEQYKQKIFKMVDFCTTIKTTEVPDPYYGGEKGFHRVIDILEDACENLIIKLEEGK--

General information: TITO was launched using: RESULT: Template: 3JS5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 842 -103536 -122.96 -681.15 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -122.96 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_3JS5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JS5-query.scw PDB file : Tito_Scwrl_3JS5.pdb: