Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTT-INESFSVSPSWGSWGITLYTLGLSVSVPIVGKLSDRY-GRKKLFLIEVCLFGLGSLLVALSQ-SFPLFLISRLIQALGGGGIFIIGSSHILATLPKE-KQGKALGLLGAMNGMAAVLGPNIGSFLLDW--------TGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSL---SILAVMYGMTNLDGANLLHSLGNPEVYGCIIFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLS-GGLLAAVIFIPSYVEQYLGVP---------AAKAGYWMTPLALASGIGAWLGGALTDKKGP-VKTVILSGIISCAGFALFPLWV-------TEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTALGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISDAIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR 4TPH Chain:A ((16-450)) ----------------TVSLIELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEF-GWHAAFAVCCVGILVGLGN-YALMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILE-YEDVAR---VFVYAAGVAVLGIFFHL------------ERAGL---IAALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSG-LGLAMIAR--------MMGAYFVASGISQYLGGVVANFASVP---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1870 -275986 -147.59 -745.91 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -147.59 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: