Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVIPLFIIAAGLVIAGYGGFKLIDTNTKTEQTLKEAKLAAKKPQEASGTKNSTDQAKNKASFKPETGQASGILEIPKINAELPIVEGTDADDLEKGVGHYKDSYYPDENGQIVLSGHRD----TVFRRTGELEKGDQLRLLLSYGEFTYEIVKTKIVDKDDTSIITLQHEKEELILTTCYPFSYVGNAPKRYIIYGKRVT 4D70 Chain:A ((28-187)) ---------------------------SSVEKKTLEEFKEKFNYSEEE----------KKKTLEEIKNGDGIALIDIEKIGVHTVIAEGSTLDVLENNIGHFENTAMPGENGNFSIAGHRNTINNEVFRNIDKLQVGDEIKITTLTDIFQYEINEIFVTSPSDTDVLNQNLDEKTMTIVTCTNR-----GKDRYIVKAKLIG

General information: TITO was launched using: RESULT: Template: 4D70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 753 -48041 -63.80 -307.96 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -63.80 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_4D70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D70-query.scw PDB file : Tito_Scwrl_4D70.pdb: