Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTE------DASRMEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGARYDLP----HLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV---MHFDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTY-----------CDSLFDMVIHQKIGEFARSLGMKK 4I5I Chain:A ((6-262)) IEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIY----PGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPL--AIMKPEIVFFGENLPEQFHRAMKYDK-DEVDLLIVIGSSLKVRPVALIP--SSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAK------

General information: TITO was launched using: RESULT: Template: 4I5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1136 -133655 -117.65 -576.10 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -117.65 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4I5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I5I-query.scw PDB file : Tito_Scwrl_4I5I.pdb: