Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQLNGKDVKWKKDTGTIQDLLASYQLEN------KIVIVERNKEIIGKERYHEVELCDRDVIEIVHFVGGG 3RPF Chain:C ((17-74)) ---------FIK-A-NDLKELRAILQEKEGLKEWLGVCAIALNDHLID---NLNTPLKDGDVISLLPPVCGG

General information: TITO was launched using: RESULT: Template: 3RPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 114 -21248 -186.39 -408.62 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -186.39 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_3RPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RPF-query.scw PDB file : Tito_Scwrl_3RPF.pdb: