Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKHNWTLETQLVH-NPFKTDGGTGAVSVPIQHASTFHQSSFEEFG--------AYDYSRSGTPTRTALEETIAALEGGTRGFAFSSGMAAIS-TAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAVVQLA-KENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGK-QLYKLQNAFGAVLGVQDCWLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGAVLSFELESK-EAVKKLVENVSLPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGLLRLSVGVEHADDLEHDFEQALKEIAPVSVR 1E5F Chain:B ((4-392)) ---ERMTPATACIHANPQKDQ--FGAAIPPIYQTSTFVFDNCQQGGNRFAGQESGYIYTRLGNPTVSNLEGKIAFLEKTEACVATSSGMGAIAATVLTILKAGDHLISDECLYGCTHALFEHALTKFGIQVDFINTAIPGEVKKHMKPNTKIVYFETPANPTLKIIDMERVCKDAHSQEGVLVIADNTFCSPMITNPVDFGVDVVVHSATKYINGHTDVVAGLICGKADLLQQIRMVGIKDITGSVISPHDAWLITRGLSTLNIRMKAESENAMKVAEYLKSHPAVEKVYYPGFEDHEGHDIAKKQMRMYGSMITFILKSGFEGAKKLLDNLKLITLAVSLGGCESLIQHPASMTHAVVPKEEREAAGITDGMIRLSVGIEDADELIADFKQGL---------

General information: TITO was launched using: RESULT: Template: 1E5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2250 -229560 -102.03 -610.53 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -102.03 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_1E5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5F-query.scw PDB file : Tito_Scwrl_1E5F.pdb: