Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLH--PEADLISGDVKPFSMVEGLDDTFRGALFLGYHARAST-PGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAGDDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKAGRLL---TEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEIKTGTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC 2X7J Chain:A ((34-182)) --------------------------------------IGSFIDEFALSGITDAVVCPGSRST--PLAVLCAAHPDISVH----------VQIDERSAGFFALGLAKAKQRPVLLICTSGTA-AANF-YP-------A--VVEAHYSRVPIIVLTADRPHELREVGAPQAINQHFLFGNFVKFFTDSALPEESPQMLRYIRTLASRAAGE----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2X7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 651 -87879 -134.99 -614.53 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -134.99 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_2X7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X7J-query.scw PDB file : Tito_Scwrl_2X7J.pdb: