Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKPFIICDFDGTITMNDNIINIMKTFAPPEWMALKDGVLSKTLSIKEGVGRMFGLLPSS-LKEEITSFVLEDAKIREGFREFVAFINEHEIPFYVISGGMDFFVYPLLEGIVEKDRIYCNHASFDNDYIHIDWPHSCKGTCSNQCGCCKPSVIHELSEPNQYIIMIGDSVTDVEAAKLSDLCFA---RDYLLNECREQNLNHLPYQDFYEIRKEIENVKEVQEWLQNKNAGESSLK 1RKU Chain:A ((1-206)) ---DMEIACLDLEGVL-----VPEIWIAFAEKTGIDALKATTRDIPDYDVLMKQRLRILDEHGLKLGDIQEVIATLKPLEGAVEFVDWLRER-FQVVILSDTFYEFSQPLMRQL-GFPTLLCHKLEIDDSDRVVGYQLRQKD--------PKRQSVIAFKSLYYRVIAAGDSYNDTTMLSEAHAGILFHAPENVIREFPQFP--------------AVHTYEDLKREFLKASSRSLSL-

General information: TITO was launched using: RESULT: Template: 1RKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 947 -146032 -154.20 -722.93 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -154.20 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_1RKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RKU-query.scw PDB file : Tito_Scwrl_1RKU.pdb: