Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLQSDQLLEATVGQFM-IEADKVAHVQVGNNLEHALLVLTKTGYTAIPVLDPSYRLHGLIGTNMIMNSIFGLERIEFEKLDQITVEEV-MLT-DIPRLHINDPIMKGFGMVINNGF--VCVENDEQVFEGIFTRRVVLKELNKHIRSLNK 2NYC Chain:A ((182-320)) -------HFLKIPIGDLNIITQDNMKSCQMTTPVIDVIQMLTQGRVSSVPIIDENGYLINVYEAYDVLGLIKGG--------LSLSVGEALMRRS--YTCTKNDKLSTIMDNIRKARVHRFFVVDDVGRLVGVLTLSDILKYILLG------

General information: TITO was launched using: RESULT: Template: 2NYC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 498 -85303 -171.29 -687.93 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -171.29 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_2NYC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NYC-query.scw PDB file : Tito_Scwrl_2NYC.pdb: