Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLIKLEQPNCNPCKMVSNYLEQVNIQFET------VDVTQEPEVAARFGVMGVPVTILLSDQGEEVNRSVGFKPNELDELLKELR 3O6T Chain:C ((31-110)) --LVDFWATWCGPSKMVAPVLEEIATERATDLTVAKLDVDTNPETARNFQVVSIPTLILFKD-GQPVKRIVGAKGKA--ALLREL-

General information: TITO was launched using: RESULT: Template: 3O6T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 256 5941 23.21 80.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : 23.21 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_3O6T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O6T-query.scw PDB file : Tito_Scwrl_3O6T.pdb: