Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------MSMTVEQMTKVFRLVMDDVELNRLLYYKTD--P-LSPSHPDVQSLENYYDSTNDSPAIINTIFKRAPKTDDLSDSPLCRMCIYLGNALPKPTNQSFMLLNQELMIDVYTHINTFEISEYRSLK-IIDRVSKL-FFNKNIAGFGVTVDYKRLLISNPPDGYLGYKMIFTFGASK----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4HVM Chain:A ((12-467)) GLRRASFLQRGAWRWLREAPPAAAFAARGLLGSGRIDDDRLAAAADEVLDAFPLLRVNFVDDDGLWMRTRENADALVRSDLRGHPDPQARCVELLRADRDRPTDPERDPLVRLHLVR-------------LSETDVVLGVVAH------------QMLLDARSRYMVLGAVWQAY-----YGRFR----PAQYRDFAEVADFHPLDRETVRVARHRWWSRRLPALPVRGPPETSRLRVPGSRWQALTEPNGSLAMAALTAWWLWTQDSLYLSTEVDLRDHLQLGSVVGPLTDRVVFGVDLTGLREPSFRDLMSRTQAGFLDAVVHYLPYHDVVDLAVDLGVVTPPRVAARWDVAVHLVSIELFREADLIGDTWDGTDTWDGTTTDLSVGELGEDMVIVLDQRRSALLDGLDAAMAQAVADPSAPLPH

General information: TITO was launched using: RESULT: Template: 4HVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 -25991 -46.83 -193.96 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -46.83 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_4HVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HVM-query.scw PDB file : Tito_Scwrl_4HVM.pdb: