Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEKAEGTTLVVVNSVCG-CAAGLARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGQEPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC 3WGD Chain:A ((6-111)) -------------------------KHLYTADMFTHGIQ-SAAHFVMFFAPWCGHCQR--LQPTWNDLGDK-YNSMEDAKVYVAKVDCTAHSDVCSAQG--VRGYPTLKLFKPGQEAVKY----QGPRDFQTLENWMLQTL----

General information: TITO was launched using: RESULT: Template: 3WGD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 436 -7416 -17.01 -70.63 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -17.01 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_3WGD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WGD-query.scw PDB file : Tito_Scwrl_3WGD.pdb: