Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFN-----------IQTSDRMKELIDVLYERFD-DLSEDT----KKAVELAKKEYEENKK---------IPEAEYKEY--VILCSKAETAWEEAKGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEAIIPLVKQVTASGN---KP-------DTSF---------------------I------------TKAFPKEKQKELSLYFLQELGYDF-D--------GGRL--DE--TVHPFATTLNR-GDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPYYKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTD--A--EGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKMLEDLP---EFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGEE-LNVRYLIDYLSNKYSNLYLL 4C2O Chain:A ((7-564)) ----EASKFVEEYDRTSQVVWNEYAEANWNYNTNITTETSKILLQKNMQIANHTLKYGTQARKFDVNQLQNTTIKRIIKKVQDLERAALPAQELEEYNKILLDMETTYSVATVCHPNGSCLQLEPDLTNVMATSRKYEDLLWAWEGWRDKAG-RAILQFYPKYVELINQAARLNGY-VDAGDSWRSMYETPSLEQDLERLFQELQPLYLNLHAYVRRALHRHYGAQHINLEGPIPAHLLGNMWAQTWSNIYDLVVPFPSAPSMDTTEAMLKQGWTPRRMFKEADDFFTSLGLLPVPPEFWNKSMLEKPTDGREVVCHASAWDFYNGKDFRIKQCTTV--NLEDLVVAHHEMGHIQYFMQYK-DL-PVALREGANPGFHEAIGDVLALS-VSTPKHLHSLN-LLSSEGG-----S-DEHDI---NF----LMKMALDKIAFIPFSYLVTQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRTQGDFDPGAKFHIPS--SVPYIRYFVSFIIQFQFHEALCQAAGHTGPLHKCDI-YQSKEAGQRLA-TAMKLGFSRPWPEAMQLITGQPNMSASAMLSYFKPLL------

General information: TITO was launched using: RESULT: Template: 4C2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2047 40990 20.02 87.96 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 20.02 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_4C2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C2O-query.scw PDB file : Tito_Scwrl_4C2O.pdb: