Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKLTSFLADRKKTIENQLSVYTEKLDMPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAYGKS---EKDGIPVGCAVEMIHTYSLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQVADMEAENRQVTLEELESIHERKTAKLLGFCVIAGAILADAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVGSDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGL--SLQKDLLYELCDLIAARDH 3M9U Chain:C ((36-286)) --------------------------------LAAAMTYSVLAGGKRLRPLLTVATMQSLGVTFVPERHWRPV-MALELLHTYSLIHDDLPAMDNDALRRGEPTNHVKFGAGMATLAGDGLLTLAFQWLTA---TDLPATMQAALVQALATAAGPSGMVAGQAKDIQSEHVNLPLSQLRVLHKEKTGALLHYAVQAGLILGQAPEAQWPAYLQFADAFGLAFQIYDDILDVVSS--------------AKNTYPGKLGLIGANQALIDTIHSGQAALQGLPTSTQRD--------------

General information: TITO was launched using: RESULT: Template: 3M9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1019 -16131 -15.83 -69.53 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -15.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_3M9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M9U-query.scw PDB file : Tito_Scwrl_3M9U.pdb: