Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIATEAVMKQEVYQVNGMNLWYGQHHALKNINLSIYENEVTAIIGPSGCGKSTFIKTLNLMIQMTPNVKLAGELNYNGSNILKDKVDIVDLRKNIGMVFQKGNPFPQ-SIFDNVAYGPRVHGTKNKKKLQEIVEKSLKDVALWDEV--KDRLHTSALSLSGGQQQRLCIARALATNPDILLMDEPTSALDPISTRKIEELILELKDK-YTIVIVTHNMQQAARVSDQTAFFYMGELVECDNTNKMFSNPKDQRTLDYISGKFG 2OLK Chain:C ((34-263)) ----------------------FGSLEVLKGINVHIREGEVVVVIGPSGSGKSTFLRCLNLLEDFD-----EGEIIIDGINLKAKDTNLNKVREEVGMVFQRFNLFPHMTVLNNITLAP----MKVRKWPREKAEA--KAMELLDKVGLKDKAHAYPDSLSGGQAQRVAIARALAMEPKIMLFDEPTSALDPEMVGEVLSVMKQLANEGMTMVVVTHEMGFAREVGDRVLFMDGGYIIEEGKPEDLFDRPQHERTKAFLSKVF-

General information: TITO was launched using: RESULT: Template: 2OLK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1133 -38867 -34.30 -171.98 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -34.30 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_2OLK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLK-query.scw PDB file : Tito_Scwrl_2OLK.pdb: