Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEFMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTDAPFQINVFVPLGLEMPPKDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRGAFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLICHESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTL--PYP--YQNTLTKAMRQKASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQEDIKI 2Z6I Chain:A ((2-308)) KTRITELLKIDYPIFQGGMA-WVADGDLAGAVSKAGGLGIIGGGNAPKEVVKANIDKIKSLTDKPFGVNIMLLSPF---------------------------------VEDIVDLVIEEGVKVVTTGAGNP-SKYMERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGK---------LTTMTLVRQVATAISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDTTISAQHFGHAVRAIKNQLTRDFELAEKDAFKQ-DLEIFEQMGAGALAKAVV-HGDVDGGSVMAGQIAGLVSKEETAEEILKDLYYGAAK-

General information: TITO was launched using: RESULT: Template: 2Z6I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1909 -160559 -84.11 -535.20 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -84.11 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_2Z6I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z6I-query.scw PDB file : Tito_Scwrl_2Z6I.pdb: