Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAISLAVDQFVAVLTIHNPPA-NALSSRILEELSSCLDQCETDAGVRSIIIHGEGRFFSAGADIKEFTSLKGNE--DSSLLAERGQQLMERIESFPKPIIAAIHGAALGGGLELAMACHIRIAAEDAKLGLPELNLGIIPGFAGTQRLPRYVGTAKALELIGSGEPISGKEALDLGLVS--IGAKDEAEVIEKAKALAAKFAEKSPQTLASLLELLYSNKVYSYEGSLK----LEAKRFGEAFESEDAKEGIQAFLEKRKPQFKGE 3Q0J Chain:D ((12-255)) --------DQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKEMADLTFADAFTADFFATWG-----KLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD---LLTEARATATTISQMS----ASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQSEGMAAFIEKRAPQF---

General information: TITO was launched using: RESULT: Template: 3Q0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1140 -14333 -12.57 -60.99 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -12.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_3Q0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q0J-query.scw PDB file : Tito_Scwrl_3Q0J.pdb: