Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYECIQDIFGGLKNPSVKDLATSLKQIPNAAKLSQPYIKEPDQYAYGRNAIYR-NNELEIIVINIPPNKETTVHDHGQSIGCAMVLEGKLLNSIYRSTGE---HAELSNSYFVHEGECLIS-TKGLIHKMSNPT-SERMVSLHVYSPPLEDMTVFEEQKEVLENS 4IEU Chain:A ((13-176)) ADLIRILHELFAG-DEVNVEEVQAVLEAYESNPAEWALYAKFDQYR-YTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKKSERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNK-

General information: TITO was launched using: RESULT: Template: 4IEU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 753 -13149 -17.46 -83.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -17.46 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_4IEU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IEU-query.scw PDB file : Tito_Scwrl_4IEU.pdb: