Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIFQRTIVVLIGTQLAASAVILFIFDLNSYNHFSGSF-SWLHFLKELAGSFAFYLFSAGLFFLLIGLCAPSRKKKRISVHEKENSLK 2ZEJ Chain:A ((80-119)) ------------------RALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVG------------------------------

General information: TITO was launched using: RESULT: Template: 2ZEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 71 -18037 -254.04 -462.49 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -254.04 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_2ZEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZEJ-query.scw PDB file : Tito_Scwrl_2ZEJ.pdb: