Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGATVHELAGVEPNPRVSTVNKGVAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDG-DAWDIVTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEKYGWGSPLVFPKFSILDPVNTFTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYELRETILYTGTIALNGMLSMG-ARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSENPARMKQLAVRVFDVEEAGKTDEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIADKAMANG-TFGQFKSLNKEDVLSILKASL 1O2D Chain:A ((17-369)) ------YMPTDVFFGEKILEKRGNIIDLLGKRALVVTGKSSSKKNGSLDDLKKLLDETEISYEIFDEVEENPSFDNVMKAVERYRNDSFDFVVGLGGGSPMDFAKAVAVLLKEKDLSVEDLYDRE-KVKHWLPVVEIPTTAGTGSEVTPYSILTDPE-GNKRGC--TLMFPVYAFLDPRYTYSMSDELTLSTGVDALSHAVEGYLSRKST-PPSDALAIEAMKIIHRNLPKAIEG--NREARKKMFVASCLAGMVIAQTGTT----LAHALGYPLTTEKGIKHGKATGMVLPFVMEVMKEEIPEKVDTVNHI-FGG----------------SLLKFLKELGLYEKV---AVSSEELEKWVEKGSRAKHLKNTPGTFTPEKIRNIYREAL

General information: TITO was launched using: RESULT: Template: 1O2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2160 -82980 -38.42 -237.08 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -38.42 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_1O2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O2D-query.scw PDB file : Tito_Scwrl_1O2D.pdb: