Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFVDRSKIEKTLGFFEHQLALFDSQTD---WQSEIGELALQRIGHLLIECILDTGNDMIDGFIMRDPGSYDDIMDILVDEKVVTEKEGDELKKLIAYRKTLVQQYLLADSGELYRLIKAHQTALQDFPKRIRSYLETELGPVSAFK 1DWN Chain:A ((1-127)) -----SKTIVLSVGEATRTLTEIQSTADRQIFEEKVGPL----VGRLRLTASLRQNGAK-TAYRVNLKLDQADVVD--CSTSVCGELPKVRYTQVWSHDVTIVANSTEASRKSLYDLTKSLV------ATSQVEDLVVNLVPLGR--

General information: TITO was launched using: RESULT: Template: 1DWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 339 7908 23.33 63.77 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 23.33 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.060

(partial model without unconserved sides chains): PDB file : Tito_1DWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DWN-query.scw PDB file : Tito_Scwrl_1DWN.pdb: