Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTKILKAPDGSPSDVERIKKESDYLRGT--LKEVMLDRISAGIPDDD-NRLMKHHGSYLQDDRDLRN---ERQKQKLEPAYQFMLRVRMPGGVSTPEQWLVMDDLSQKYGNGTLKLTTRETFQMHGILKWNMKKTIQTIHSALLDTIAACGDVNRNVMCASNPYQSEI-HSEVYEWSKKLSDDLLPRTRAYHEIWLDEERVAGTPEEEVEPMYGPLYLPRKFKIGIAVPPSNDIDVFSQDLGFIAIVEDGKLIGFNVAIGGGMGMTHGDTATYPQLAKVI-GFCRPEQMYDVAEKTITIQRDYGNRSVRKNARFKYTVDRLGLENVKEELENR-LGWSLEE-AKPYHFDHNGDRYGWVEGIEDKWHFTLFVEGGRITDYDDYKLMTGLREIAKVHT-GEFRLTANQNLMIANVSSDKKEEISALIEQYGLTDGKHYSALRRSSMACVALPTCGLAMAEAERYLPTLLDKIEEIIDENG-LRDQEITIRMTGCPNGCARHALGEIGFIGKAP--------GKYNMYLGAAFDG-SRLSKMYR-ENIGEADILSELRILLSRYAKEREEGEHFGDFVIRAGIIKATTDGTNFHD 1ZJ8 Chain:A ((32-565)) ----------EPLNANEELKKAGNPLDVRERIENIYAKQGFDSIDKTDLRGRFRWWGLYTQREQGYDGTWTGDDNIDKLEAKYFMMRVRCDGGALSAAALRTLGQISTEFARDTADISDRQNVQYHWIEVENVPEIWRRLDDVGLQTTEACGDCPRVVLGSPLAGESLDEVLDPTWAIEEIVRRYIG-KPDF------------------------ADLPRKYKTAISGLQD--VAHEINDVAFIGVNHPEHGPGLDLWVGGGLSTN-------PMLAQRVGAWVPLGEVPEVWAAVTSVFRDYGYRRLRAKARLKFLIKDWGIAKFREVLETEYLKRPLIDGPAPEPVKHPIDHVGVQRLKNGLNAVGVAPIAGRVSG----TILTAVADLMARAGSDRIRFTPYQKLVILDIPDALLDDLIAGLDALGLQS--RPSHWRRNLMACSGIEFCKLSFAETRVRAQHLVPELERRLEDINSQLDVPITVNINGCPNSCARIQIADIGFKGQMIDDGHGGSVEGFQVHLGGHLGLDAGFGRKLRQHKVTSDELGDYIDRVVRNFVKHRSEGERFAQWVIRAEEDDL---------

General information: TITO was launched using: RESULT: Template: 1ZJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3130 70107 22.40 136.93 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 22.40 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_1ZJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZJ8-query.scw PDB file : Tito_Scwrl_1ZJ8.pdb: